mgi technologies dnbseq g400  (Complete Genomics Inc)

Journal: Nature Biotechnology

Article Title: Efficient hybrid de novo assembly of human genomes with WENGAN

doi: 10.1038/s41587-020-00747-w

Figure Lengend Snippet: a , The de novo assemblies were sorted by NG50 at each long-read coverage (lolliplot). We computed the NGA50 (which corresponds to the NG50 corrected of assembly errors) of each assembly using QUAST (see ). b , The consensus quality (see ) of each genome assembly and the CPU hours required for the assembly. c , The WENGAN (W- X ) and FLYE assemblies of the complex MHC region located in Chr6: 28,477,797–33,448,354 (4.97 Mb). The MHC sequence was aligned to the genome assemblies and the aligned blocks ≥30 kb with a minimum identity of 95% were kept. The alignment breakpoints (vertical black lines) indicate a contig switch, an alignment error or a gap in the assembly. The assemblies of the MHC region are displayed in tracks by long-read coverage.

Article Snippet: Moreover, we assessed the suitability of the MGI sequencing technology (MGISEQ-2000) as an alternative to Illumina SBS for hybrid assembly using matched short-read genomic data.

Techniques: Sequencing